control short hairpin rna shctrl  (Santa Cruz Biotechnology)

Journal: Journal of Virology

Article Title: Disassembly of the TRIM23-TBK1 Complex by the Us11 Protein of Herpes Simplex Virus 1 Impairs Autophagy

doi: 10.1128/JVI.00497-19

Figure Lengend Snippet: (A) TRIM23 depletion in human lung fibroblasts (HEL). Lysates of cells that were transduced with control shRNA (shCtrl) or shRNA targeting TRIM23 (shTRIM23) were subjected to Western blot analysis with antibodies against TRIM23 and β-actin. (B) Conversion of LC3B-I to LC3B-II. HEL cells, expressing shCtrl or shTRIM23, were mock infected, infected with wild-type HSV-1, Δγ134.5, or EUs11 (MOI of 5) for 12 h, or incubated with rapamycin (200 nM) for 10 h. Cell lysates were processed for Western blot analysis with antibodies against LC3B, US11, ICP27, and β-actin. (C) Quantification of LC3B-II/LC3B-I. The protein bands shown in panel B were quantified using NIH ImageJ software and are presented as the amount of LC3B-II relative to that of LC3B-I, normalized to the total amount of β-actin in each sample. The data are averages from three independent experiments and were statistically assessed by a two-tailed Student's t test. **, P < 0.01.

Article Snippet: HEL cells stably expressing control short hairpin RNA (shCtrl) or TRIM23-specific short hairpin RNA (shTRIM23) were produced through lentiviral transduction and selection with puromycin (Santa Cruz).

Techniques: Transduction, Control, shRNA, Western Blot, Expressing, Infection, Incubation, Software, Two Tailed Test